Kinetic Analysis of a Chitinase from Red Sea Bream, Pagrus major

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著者

    • SAKUDA Shohei
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • KOGA Daizo
    • Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University

抄録

Kinetic analysis was done on the 46-kDa chitinase (EC 3.2.1.14) purified from the stomach of red sea bream, <I>Pagrus major</I>, using glycolchitin and <I>N</I>-acetylchitooligosaccharides (GlcNAc<SUB>n</SUB>, <I>n</I>=2–6) as substrates. High activity was observed at two pHs, such as 2.5 and 9.0, toward glycolchitin as seen in other insect chitinases, and also at both pH 2.5 and 5.0 even toward a short substrate, <I>N</I>-acetylchitopentasaccharide. Allosamidin competitively inhibited chitinase with <I>K</I>i value of 0.0214 μ<small>M</small> at pH 2.5 and 0.0024 μ<small>M</small> at pH 9.0 in the reaction of glycolchitin. Substrate inhibition was observed in the reaction of <I>N</I>-acetylchitopentasaccharide. The anomeric forms of the products from <I>N</I>-acetylchitooligosaccharides were analyzed to be β anomer by the high pressure liquid chromatography (HPLC) method. The data for both β-anomer formation and allosamidin inhibition suggest that red sea bream chitinase belongs to family 18 of glycosyl hydrolases. This suggestion is also supported by the results for the N-terminal amino acid sequence.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 68(6), 1338-1344, 2004-06-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

参考文献:  33件中 1-33件 を表示

被引用文献:  4件中 1-4件 を表示

各種コード

  • NII論文ID(NAID)
    10013286813
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    6991184
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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