Functional analyses of lipocalin-type and hematopoietic prostaglandin D synthases.

• URADE Yoshihiro

  Department of Molecular Behavioral Biology, Osaka Bioscience Institute

• EGUCHI Naomi

  Department of Molecular Behavioral Biology, Osaka Bioscience Institute

• ARITAKE Kosuke

  Department of Molecular Behavioral Biology, Osaka Bioscience Institute

• HAYAISHI Osamu

  Department of Molecular Behavioral Biology, Osaka Bioscience Institute

Bibliographic Information

Other Title

• リポカリン型および造血器型プロスタグランジンＤ合成酵素の機能解析
• リポカリンガタ オヨビ ゾウケツキガタ プロスタグランジン D ゴウセイ コウソ ノ キノウ カイセキ

Abstract

Prostaglandin (PG) D synthase (PGDS) catalyzes the isomerization of PGH₂ to PGD₂, which acts as an endogeous somnogen and an allergic mediator. There are two distinct types of PGDS: one is lipocalin-type PGDS (L-PGDS) localized in the central nervous system, male genitals, and heart; and the other is hematopoietic PGDS (H-PGDS) in mast cells and Th2 lymphocytes. L-PGDS is the same as beta-trace, a major protein in human cerebrospinal fluid, and is also secreted into the seminal plasma and plasma. The L-PGDS concentration in various body fluids is useful as a marker for various diseases such as renal failure and coronary atherosclerosis. H-PGDS is a cytosolic enzyme and is a member of the Sigma class of glutathione S-transferase. We determined the X-ray crystallographic structures of H-PGDS and L-PGDS. We also generated the gene-knockout (KO) mice and the human enzyme-overexpressing transgenic mice for each PGDS. L-PGDS-KO mice lacked PGE₂-induced tactile allodynia and rebound of non-rapid eye movement sleep after sleep deprivation. Human L-PGDS-overexpressing transgenic mice showed an increase in non-rapid eye movement sleep due to accumulation of PGD₂ in the brain after tail clipping. H-PGDS-KO mice showed an allergic reaction weaker than that of the wild-type mice.<br>

Journal

• Folia Pharmacologica Japonica

  Folia Pharmacologica Japonica 123 (1), 5-13, 2004

  The Japanese Pharmacological Society

Keywords

• sleep
• allergy
• clinical marker protein
• x-ray crystallography
• gene-manipulated mice

Details

• CRID: 1390001204271379072
• NII Article ID: 10013289896
• NII Book ID: AN00198335
• DOI: 10.1254/fpj.123.5
• ISSN: 13478397
• ISSN: 00155691
• NDL BIB ID: 6812358
• PubMed: 14695453
• Web Site: http://id.ndl.go.jp/bib/6812358
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6812358
• Web Site: https://search.jamas.or.jp/link/ui/2004217496
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed