JunD-Menin Interaction Regulates c-Jun-mediated AP-1 Transactivation

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著者

    • IKEO YASUTO
    • Department of Aging Medicine and Geriatrics, Shinshu University Graduate School of Medicine
    • YUMITA WATARU
    • Department of Aging Medicine and Geriatrics, Shinshu University Graduate School of Medicine
    • SAKURAI AKIHIRO
    • Division of Medical Genetics, Department of Preventive Medicine, Shinshu University School of Medicine
    • HASHIZUME KIYOSHI
    • Department of Aging Medicine and Geriatrics, Shinshu University Graduate School of Medicine

抄録

The gene responsible for multiple endocrine neoplasia type 1, <i>MEN1</i>, encodes the 610-amino acid-protein, menin. Although menin has been reported to bind AP-1 transcription factor JunD and suppress its transcriptional activity, little is known about its molecular mechanisms and physiological role. To better understand the function of menin and its significance in tumorigenesis, we investigated the effect of wild-type and mutant menin proteins on AP-1 transactivation. In COS cells, wild-type menin suppressed JunD-mediated transactivation in a dose-dependent manner, while it augmented c-Jun-mediated transactivation also in a dose-dependent manner. These effects were lost or reduced in all menin mutants examined. Electrophoretic mobility shift assay using AP-1 binding elements as a probe revealed that menin does not affect binding of c-Jun to DNA. Coexpression of menin mutants did not affect the function of wild-type menin. Coexpression of JunD amino-terminal fragment abolished menin-mediated enhancement of c-Jun transactivation, suggesting that Menin-JunD interaction may negatively regulate the enhancing effect of menin on c-Jun-mediated transactivation in COS cells.<br>

収録刊行物

  • Endocrine journal

    Endocrine journal 51(3), 333-342, 2004-06-01

    一般社団法人 日本内分泌学会

参考文献:  38件中 1-38件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10013292992
  • NII書誌ID(NCID)
    AA10901436
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09188959
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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