Annexin 5 Inhibits Thyrotropin Releasing Hormone (TRH) Stimulated Prolactin Release in the Primary Culture of Rat Anterior Pituitary Cells

• KAWAMINAMI Mitsumori

  Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• SENDA Akio

  Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• ETOH Seishi

  Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• MIYAOKA Hiroko

  Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• KURUSU Shiro

  Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• HASHIMOTO Inoru

  Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

抄録

Annexin 5, a novel calcium-phospholipid binding protein, is thought to be involved in hormone secretion by the anterior pituitary gland. Gonadotropin releasing hormone stimulates annexin 5 synthesis, which, in turn, enhances gonadotoropin secretion. On the other hand, annexin 5 was shown to inhibit prolactin release in vitro. To understand the nature of the opposing effects of annexin 5 on these two major pituitary hormones, the present study examines the inhibitory effect of annexin 5 on prolactin release in relation to thyrotropin stimulating hormone (TRH) using primary cultures of anterior pituitary cells of adult female rats. While recombinant rat annexin 5 was found to have little effect on basal prolactin release, it significantly inhibited TRH-stimulated prolactin release. Addition of specific anti-annexin 5 serum to the culture increased basal prolactin release in a concentration dependent manner, and no further increase in prolactin release was observed following application of TRH in the presence of anti-annexin 5. The enhanced basal prolactin release induced by anti-annexin 5 was reversed by the simultaneous administration of indomethacin, an inhibitor of cyclooxygenase. These results demonstrate that endogenous pituitary annexin 5 exerts an inhibitory effect on prolactin release and suggest that this is attained by suppression of eicosanoid synthesis in vitro.<br>

収録刊行物

• Endocrine Journal

  Endocrine Journal 51 (3), 349-354, 2004

  一般社団法人 日本内分泌学会

キーワード

• Annexin 5
• Prolactin
• Pituitary
• TRH
• Indomethacin

詳細情報

• CRID: 1390001206300499328
• NII論文ID: 10013293048
• NII書誌ID: AA10901436
• DOI: 10.1507/endocrj.51.349
• ISSN: 13484540
• ISSN: 09188959
• PubMed: 15256781
• Web Site: http://www.jstage.jst.go.jp/article/endocrj/51/3/51_3_349/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005050377
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可