Annexin 5 Inhibits Thyrotropin Releasing Hormone (TRH) Stimulated Prolactin Release in the Primary Culture of Rat Anterior Pituitary Cells

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著者

    • KAWAMINAMI MITSUMORI
    • Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • SENDA AKIO
    • Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • ETOH SEISHI
    • Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • MIYAOKA HIROKO
    • Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • KURUSU SHIRO
    • Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • HASHIMOTO INORU
    • Laboratory of Veterinary Physiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

抄録

Annexin 5, a novel calcium-phospholipid binding protein, is thought to be involved in hormone secretion by the anterior pituitary gland. Gonadotropin releasing hormone stimulates annexin 5 synthesis, which, in turn, enhances gonadotoropin secretion. On the other hand, annexin 5 was shown to inhibit prolactin release <i>in vitro</i>. To understand the nature of the opposing effects of annexin 5 on these two major pituitary hormones, the present study examines the inhibitory effect of annexin 5 on prolactin release in relation to thyrotropin stimulating hormone (TRH) using primary cultures of anterior pituitary cells of adult female rats. While recombinant rat annexin 5 was found to have little effect on basal prolactin release, it significantly inhibited TRH-stimulated prolactin release. Addition of specific anti-annexin 5 serum to the culture increased basal prolactin release in a concentration dependent manner, and no further increase in prolactin release was observed following application of TRH in the presence of anti-annexin 5. The enhanced basal prolactin release induced by anti-annexin 5 was reversed by the simultaneous administration of indomethacin, an inhibitor of cyclooxygenase. These results demonstrate that endogenous pituitary annexin 5 exerts an inhibitory effect on prolactin release and suggest that this is attained by suppression of eicosanoid synthesis <i>in vitro</i>.<br>

収録刊行物

  • Endocrine journal

    Endocrine journal 51(3), 349-354, 2004-06-01

    一般社団法人 日本内分泌学会

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各種コード

  • NII論文ID(NAID)
    10013293048
  • NII書誌ID(NCID)
    AA10901436
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09188959
  • データ提供元
    CJP書誌  J-STAGE 
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