Reversal of Streptozotocin-induced Hyperglycemia by Transplantation of Pseudoislets Consisting of β Cells Derived from Ductal Cells

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著者

    • OGATA TAKEKI
    • Institute for Molecular & Cellular Regulation, Gunma University
    • PARK KEE YOUNG
    • Institute for Molecular & Cellular Regulation, Gunma University
    • SENO MASAHARU
    • Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University
    • KOJIMA ITARU
    • Institute for Molecular & Cellular Regulation, Gunma University

抄録

The present study was conducted in an attempt to treat streptozotocin (STZ)-induced hyperglycemia by transplanting β cells derived from pancreatic ductal cells. Ductal cells obtained from neonatal rats were cultured <i>in vitro</i>. Approximately 70% of the cells were converted to insulin-secreting cells by incubating with betacellulin and activin A. Differentiated cells responded to a depolarizing concentration of potassium, tolbutamide and a high concentration of glucose, and insulin secretion increased by 2.5-, 2.3- and 1.6-fold, respectively. We then prepared pseudoislets using the differentiated cells, which exhibited greatly improved glucose-responsiveness, with a high concentration of glucose inducing a 3-fold increase in insulin secretion. We transplanted these pseudoislets into the portal vein of STZ-treated nude mice. Before transplantation, the plasma glucose concentration was above 400 mg/dl, and after transplantation it was markedly reduced, the effect of which persisted for two weeks. These results indicate that STZ-induced hyperglycemia can be treated by transplanting pseudoislets consisting of β cells derived from ductal cells.<br>

収録刊行物

  • Endocrine journal

    Endocrine journal 51(3), 381-386, 2004-06-01

    The Japan Endocrine Society

参考文献:  13件中 1-13件 を表示

被引用文献:  5件中 1-5件 を表示

各種コード

  • NII論文ID(NAID)
    10013293163
  • NII書誌ID(NCID)
    AA10901436
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    09188959
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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