Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum

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A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150–200 <I>μ</I>m, and a porosity of 28% that was dependent on the PMMA beads quolity. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 10<SUP>7</SUP> cells·m<I>l</I><SUP>–1</SUP>-HAp. This value is similar to most existing suitable porous microcarriers.<BR>A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

収録刊行物

  • Journal of chemical engineering of Japan

    Journal of chemical engineering of Japan 37(4), 531-535, 2004-04-01

    公益社団法人 化学工学会

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各種コード

  • NII論文ID(NAID)
    10013340136
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • NDL 記事登録ID
    6929645
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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