Solid-phase Peptide Synthesis in a Microfluidic Device

• MARUYAMA Tatsuo

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• UCHIDA Jun-ichi

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• OHKAWA Tomohiro

  Tokyo Research Laboratories, Tosoh Corporation

• KUBOTA Fukiko

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• KAMIYA Noriho

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• GOTO Masahiro

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

Bibliographic Information

Other Title

• 「マイクロ化学工学」マイクロ流体デバイスを用いたオリゴペプチドの固相合成
• マイクロ流体デバイスを用いたオリゴペプチドの固相合成
• マイクロ リュウタイ デバイス オ モチイタ オリゴペプチド ノ コソウ ゴウセイ

Abstract

The solid-phase synthesis of oligopeptide was achieved for the first time in a microfluidic device. We fabricated a microchannel (width 300 μm, depth 160 μm)with a bead trap on a glass plate. Resin beads were loaded in the bead trap for the solid-phase peptide synthesis, and the synthesis of pentapeptide (Met-enkephalin) was conducted in the microchannel. The yield of the synthesized pentapeptide was confirmed to be 20% by MALDI-TOF MS and HPLC analyses.

Journal

• KAGAKU KOGAKU RONBUNSHU

  KAGAKU KOGAKU RONBUNSHU 30 (2), 180-182, 2004

  The Society of Chemical Engineers, Japan

Keywords

• Microreactor
• Oligopeptide
• Peptide Synthesis
• Solid-Phase Synthesis

Details

• CRID: 1390282679486169728
• NII Article ID: 10013341286
• NII Book ID: AN00037234
• DOI: 10.1252/kakoronbunshu.30.180
• ISSN: 13499203
• ISSN: 0386216X
• NDL BIB ID: 6913041
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6913041
• Web Site: http://www.jstage.jst.go.jp/article/kakoronbunshu/30/2/30_2_180/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed