Simultaneous Degradation of 4-Nitrophenol and Picric Acid by Two Different Mechanisms of Rhodococcus sp. PN1

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<I>Rhodococcus</I> sp. PN1 was isolated as a 4-nitrophenol (4-NP) degrading bacterium and can utilize 4-NP as a sole carbon, nitrogen, and energy source. This bacterium also degrades polynitrophenols such as 2,4-dinitrophenol and 2,4,6-trinitrophenol (picric acid). To elucidate the nitrophenol degradation pathways of strain PN1, a genomic library of strain PN1 was constructed using a <I>Rhodococcus</I> host-vector system and the genes encoding the pathways were screened from the library. Consequently, the gene clusters (<I>nphRA1A2</I> and <I>npdGIJ</I>) encoding 4-NP hydroxylation and picric acid reduction were obtained from the library, respectively. The recombinant <I>Rhodococcus</I> strain containing the <I>nphRA1A2</I> genes converted 4-NP into 4-nitrocatechol quantitatively, while that containing the <I>npdGIJ</I> genes transformed picric acid into its hydride Meisenheimer complex, quantitatively. Thus, it was found that strain PN1 has two quite different degradation mechanisms for nitrophenols, an oxygenation (4-NP hydroxylation) and a reduction (hydride transfer to the aromatic-ring of picric acid). Sequence analysis of the gene clusters suggested that the former encodes a member of the two component flavin diffusible monooxygenase family (NphA1 and NphA2), while the latter encodes an NADPH-dependent F<SUB>420</SUB> reductase (NpdG) and an F<SUB>420</SUB>-dependent picric acid reductase (NpdI). Simultaneous degradation of 4-NP and picric acid by strain PN1 indicated that both degradation mechanisms can work simultaneously without significant inhibition. This capability of strain PN1 may contribute to the treatment of industrial wastewaters containing many kinds of nitrophenols

収録刊行物

  • Journal of chemical engineering of Japan

    Journal of chemical engineering of Japan 36(10), 1178-1184, 2003-10-01

    The Society of Chemical Engineers, Japan

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各種コード

  • NII論文ID(NAID)
    10013414714
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • NDL 記事登録ID
    6720055
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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