The Normalization of Guinea Pig Leukocyte Fractions and Lymphocyte Subsets in Blood and Lymphoid Tissues using a Flow Cytometric Procedure

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Many hematological and immunological parameters remain unclear in the study of the guinea pig. In this study, we established the mean values of blood counts, the percentage of leukocyte fractions and lymphocyte subsets in blood and various lymphoid tissues of the guinea pig with a flow cytometric procedure using MIL4/SSC. The mean counts of WBC and RBC in the blood were lower, and MCV and MCH were higher than those of other rodents, resembling those of humans. Furthermore, the mean percentages of blood lymphocytes were smaller and that of granulocyte was larger than those of other rodents, resembling those of humans. We further established a flow cytometric procedure for lymphocyte subsets and clarified the mean percentages of T- and B-cells, CD4<sup>+</sup>-, CD8<sup>+</sup>- and MHC Class II<sup>+</sup>- T-cells, and CD4<sup>-</sup>CD8 <sup>-</sup> T-cells. The latter were morphologically larger in cell size and cytoplasm than CD4<sup>+</sup>- plus CD8<sup>+</sup> T-cells, and this subset had a significantly higher percentage in newborn animals. Furthermore, the appearance of the MHC Class II<sup>+</sup> T-cell subset was suggested to be a marker of hyper-activation of T-cells in BCG-immunized animals. Thus, both the novel flow cytometric procedure for leukocyte fractions and lymphocyte subsets, and the established normal values will be useful tools in studying guinea pigs as models of various diseases and biological phenomena.<br>

収録刊行物

  • Experimental animals

    Experimental animals 53(4), 321-329, 2004-07

    Japanese Association for Laboratory Animal Science

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各種コード

  • NII論文ID(NAID)
    10013441591
  • NII書誌ID(NCID)
    AA11032321
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13411357
  • NDL 記事登録ID
    7016994
  • NDL 雑誌分類
    ZS7(科学技術--医学)
  • NDL 請求記号
    Z54-H752
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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