２波長蛍光Ｄｉｐ分光法を用いたファーフィールド超解像顕微鏡の２点分解能評価

DOI Web Site Web Site 参考文献40件

書誌事項

タイトル別名
  • Two-Point-Separation in Far-Field Super-Resolution Fluorescence Microscopy Based on Two-Color Fluorescence Dip Spectroscopy
  • 2 ハチョウ ケイコウ Dip ブンコウホウ オ モチイタ ファーフィールド チョウカイゾウ ケンビキョウ ノ 2テン ブンカイノウ ヒョウカ

この論文をさがす

抄録

The two-point-resolution of a novel two-color far-field super-resolution fluorescence microscopy has been evaluated. This microscopy is based on a combination of two-color fluorescence dip spectroscopy and a phase modulation technique for the laser beam. The two-point-resolution of the microscopy has been evaluated by using the fluorescent beads of which the size is specified by the observation with AFM. By introducing two color lasers, the observed fluorescence image shrunk down beyond the diffraction limit. In the observation of neighboring two fluorescence beads, pronounced separation between two individual fluorescence spots has been investigated. By taking the focal depth into consideration, FWHM and profile of the fluorescence images were well reproduced. It is proved that our technique breaks the diffraction limit and the spatial resolution of the optical fluorescence microscope can be improved by 2 times higher than the diffraction limit.

収録刊行物

  • 表面科学

    表面科学 25 (8), 466-472, 2004

    公益社団法人 日本表面科学会

参考文献 (40)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ