Systematic Analysis of SNARE Molecules in Arabidopsis: Dissection of the post-Golgi Network in Plant Cells

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  • Uemura Tomohiro
    Graduate School of Biostudies, Kyoto University
  • Ueda Takashi
    Department of Biological Sciences, Graduate School of Science, University of Tokyo
  • Ohniwa Ryosuke L.
    Graduate School of Biostudies, Kyoto University
  • Nakano Akihiko
    Department of Biological Sciences, Graduate School of Science, University of Tokyo Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute
  • Takeyasu Kunio
    Graduate School of Biostudies, Kyoto University
  • Sato Masa H.
    Graduate School of Human and Environmental Studies, Kyoto University

書誌事項

タイトル別名
  • Systematic Analysis of SNARE Molecules in <i>Arabidopsis</i>: Dissection of the post-Golgi Network in Plant Cells
  • Systemic analysis of SNARE molecules in Arabidopsis: dissection of the post-golgi network in plant cells
  • Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in pant cells

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抄録

In all eucaryotic cells, specific vesicle fusion during vesicular transport is mediated by membrane-associated proteins called SNAREs (soluble N-ethyl-maleimide sensitive factor attachment protein receptors).<br> Sequence analysis identified a total of 54 SNARE genes (18 Qa-SNAREs/Syntaxins, 11 Qb-SNAREs, 8 Qc-SNAREs, 14 R-SNAREs/VAMPs and 3 SNAP-25) in the Arabidopsis genome. Almost all of them were ubiquitously expressed through out all tissues examined. A series of transient expression assays using green fluorescent protein (GFP) fused proteins revealed that most of the SNARE proteins were located on specific intracellular compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar compartment (PVC) and vacuoles, 2 in TGN/PVC/vacuoles, and 1 in TGN/PVC/plasma membrane.<br> Some SNARE proteins showed multiple localization patterns in two or more different organelles, suggesting that these SNAREs shuttle between the organelles. Furthermore, the SYP41/SYP61-residing compartment, which was defined as the TGN, was not always located along with the Golgi apparatus, suggesting that this compartment is an independent organelle distinct from the Golgi apparatus. We propose possible combinations of SNARE proteins on all subcellular compartments, and suggest the complexity of the post-Golgi membrane traffic in higher plant cells.<br>

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