The expression of src-suppressed C kinase substrate (SSeCKS) and uptake of exogenous particles in endothelial and reticular cells

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著者

    • RUNG-RUANGKIJKRAI Tilladit
    • Laboratories of Anatomy, Department of Biomedical Sciences, Hokkaido University Graduate School of Veterinary Medicine
    • FUJIKURA Daisuke
    • Laboratories of Biochemistry, Department of Biomedical Sciences, Hokkaido University Graduate School of Veterinary Medicine
    • KITAMURA Hiroshi
    • Laboratories of Biochemistry, Department of Biomedical Sciences, Hokkaido University Graduate School of Veterinary Medicine
    • SAITO Masayuki
    • Laboratories of Biochemistry, Department of Biomedical Sciences, Hokkaido University Graduate School of Veterinary Medicine
    • IWANAGA Toshihiko
    • Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine

抄録

<I>Src</I>-suppressed C kinase substrate (SSeCKS), a potent tumor suppressor, plays a role in membrane-cytoskeletal remodeling to regulate mitogenesis, cell differentiation, and motility. Our previous study showed that lipopolysaccharide (LPS) induced a selective and strong expression of SSeCKS in the vascular endothelial cells of several organs, such as hepatic sinusoids, and in the reticular cells of lymphoid organs. In the present immunocytochemical study, we determined the detailed cellular and subcellular localization of SSeCKS in mouse tissues after LPS administration, and examined the involvement of SSeCKS in the uptake of exogenous particles. SSeCKS immunoreactivity in the liver and lymph nodes was below the detectable level under normal conditions. After LPS stimulation, an intense immunoreactivity for SSeCKS became noticeable in sinusoidal endothelial cells of the liver and medullary reticular cells of the lymph node. Electron-microscopically, the immunoreactivity was localized predominantly along the cytoplasmic membrane of both cell types. These cells in normal mice incorporated a small amount of injected particles (carbon particles and latex beads), while after LPS stimulation, the uptake of particles increased in terms of the amount and extent of the uptaking sites. Endothelial cells and reticular cells without SSeCKS expression could not incorporate any particles even after LPS stimulation. The subcellular localization of SSeCKS in endothelial cells correlated with some pinocytic pits and phago-lysosomes, although a diffuse distribution of SSeCKS in the cytoplasm was also visible. Taken together, these findings indicate that SSeCKS expression in endothelial cells and reticular cells is a functional index of the reticulo-endothelial system and is involved in the uptake of particles from blood and lymph circulation.

収録刊行物

  • Archives of histology and cytology

    Archives of histology and cytology 67(2), 135-147, 2004-06-01

    国際組織細胞学会

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各種コード

  • NII論文ID(NAID)
    10013491376
  • NII書誌ID(NCID)
    AA1068990X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09149465
  • データ提供元
    CJP書誌  J-STAGE 
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