Atomic force microscopy of human metaphase chromosomes after differential staining of sister chromatids

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著者

    • KIMURA Eiji
    • Division of Microscopic Anatomy and Bio-imaging, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Sciences
    • HOSHI Osamu
    • Division of Microscopic Anatomy and Bio-imaging, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Sciences
    • USHIKI Tatsuo
    • Division of Microscopic Anatomy and Bio-imaging, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Sciences

抄録

Human metaphase chromosomes, in which 5-bromo-deoxyuridine (BrdU) had been incorporated into the DNA, were treated with the fluorescent plus Giemsa (FPG) method. Use of this method distinctly stained one of the paired sister chromatids with the Giemsa solution due to the difference in content of BrdU in the two chromatids. These chromosomes with their differential staining of sister chromatids were observed by atomic force microscopy (AFM). In the air-dried specimens, one of the paired chromatids that was stained strongly with Giemsa solution was about two times higher than the counterpart that was stained faintly with Giemsa solution. In the critical point dried chromosomes, the height of the Giemsa positive chromatid roughly matched that of the Giemsa negative counterpart. These findings imply that the arrangement of the Giemsa negative chromatid after FPG staining is fragile and easily collapses due to the surface tension of water during air-drying. At higher magnifications, the surface structure differed between Giemsa positive and negative chromatids; the Giemsa positive chromatid (i.e., unifilarly BrdU-incorporated chromatid) was composed of fibrous structures while the Giemsa negative chromatid (i.e., bifilarly BrdU-incorporated chromatid) exhibited a fine granular appearance. These structural changes in the sister chromatids are thought to arise from the ultraviolet irradiation and heating of the chromosomes during FPG staining.

収録刊行物

  • Archives of histology and cytology

    Archives of histology and cytology 67(2), 171-177, 2004-06-01

    国際組織細胞学会

参考文献:  18件中 1-18件 を表示

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各種コード

  • NII論文ID(NAID)
    10013491479
  • NII書誌ID(NCID)
    AA1068990X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09149465
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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