Intracellular Localization of a Class IV Chitinase from Yam

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著者

    • Yuki Dai [他] YUKI Dai
    • Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University
    • KOGA Daizo
    • Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University

抄録

Genomic DNA encoding a class IV chitinase was cloned from yam (<I>Dioscorea opposita</I> Thunb) leaves in previous research (<I>Biosci. Biotechnol. Biochem.</I>, <B>68</B>, 1508–1517 (2004)). But this chitinase had an additional sequence composed of eight amino acids (a C-terminal extension) at the C-terminal, compared with class IV chitianses from other plants. In order to clarify the role of this C-terminal extension in cellular localization, plants and suspension-cultured cells of <I>Nicotiana tabacum</I> were transformed with either the cloned yam class IV chitinase gene carrying the C-terminal extension or its truncated gene by the <I>Agrobacterium</I>-mediated method, and then their localization was investigated. The results suggest that the C-terminal extension of yam class IV chitinase plays a role as a targeting signal for plant vacuoles. This is the first report presenting the existence of vacuolar type class IV chitinase.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 68(7), 1518-1524, 2004-07-23

    公益社団法人 日本農芸化学会

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各種コード

  • NII論文ID(NAID)
    10013520046
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7031092
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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