Spontaneous Transformation and Its Use for Genetic Mapping in Bacillus subtilis
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- MURAYAMA Rikinori
- Laboratory of Molecular Genetics and Frontier Project “Life’s Adaptation Strategies to Environmental Changes”, Department of Life Science, College of Science, Rikkyo University
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- AKANUMA Genki
- Laboratory of Molecular Genetics and Frontier Project “Life’s Adaptation Strategies to Environmental Changes”, Department of Life Science, College of Science, Rikkyo University
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- MAKINO Yuki
- Laboratory of Molecular Genetics and Frontier Project “Life’s Adaptation Strategies to Environmental Changes”, Department of Life Science, College of Science, Rikkyo University
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- NANAMIYA Hideaki
- Laboratory of Molecular Genetics and Frontier Project “Life’s Adaptation Strategies to Environmental Changes”, Department of Life Science, College of Science, Rikkyo University
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- KAWAMURA Fujio
- Laboratory of Molecular Genetics and Frontier Project “Life’s Adaptation Strategies to Environmental Changes”, Department of Life Science, College of Science, Rikkyo University
Bibliographic Information
- Other Title
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- Spontaneous Transformation and Its Use for Genetic Mapping in<i>Bacillus subtilis</i>
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Abstract
Using a simple semi-synthetic competence and sporulation medium (CSM), we found evidence that Bacillus subtilis cells transformed in the competence phase can sporulate, indicating that genetic information acquired during the competence phase is inherited by the next generation after germination of the transformed spores. Moreover, the results from mixed cell culture experiments suggest that spontaneous genetic transformation can occur between competent cells and DNA released from lysed cells in the natural environment. We also found evidence that the spontaneous transformation system can be used for genetic mapping in B. subtilis.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (8), 1672-1680, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681452963968
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- NII Article ID
- 10013520665
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7066890
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- PubMed
- 15322350
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed