The Expression Profile of Lectin Differs from That of Seed Storage Proteins in Castanea crenata Trees
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- NAKAMURA Sachiko
- Graduate School of Science and Technology, Kobe University
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- IKEGAMI Ayako
- Faculty of Agriculture, Kobe University
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- MIZUNO Masashi
- Graduate School of Science and Technology, Kobe University
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- YAGI Fumio
- Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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- NOMURA Keiichi
- Faculty of Agriculture, Kobe University
Bibliographic Information
- Other Title
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- Expression Profile of Lectin Differs from That of Seed Storage Proteins in Castanea crenata Trees
- The Expression Profile of Lectin Differs from That of Seed Storage Proteins in<i>Castanea crenata</i>Trees
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Abstract
Using Northern blot analysis, the expression of the Japanese chestnut (Castanea crenata Sieb. et Zucc.) agglutinin (CCA) gene was compared with that of its seed storage protein (SSP) gene. After cDNA cloning of SSP, the expression profile of SSP mRNA and CCA mRNA were compared. SSP mRNA was seed-specific, while CCA mRNA was expressed in the stems and flowers (both male and female) as well as in the seeds. Whereas extracts from all organs observed using Western blot analysis exhibited positive signals, in seeds, large expressions of SSP mRNA were restricted to the late maturation and harvest stages. Levels were maintained during the dormant period. No expression was observed during the germination stage. In contrast, CCA mRNA expression was maintained at a high level during development, was at a relatively low level during dormancy, and showed subsequent high expression during germination. These results suggest that one of the physiological roles of CCA is to act as a vegetative storage protein. But since protein expression did not coincide with that of mRNA, the expression of CCA may be regulated both at the transcription and the translation levels.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (8), 1698-1705, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681453020672
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- NII Article ID
- 10013520787
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7066918
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- PubMed
- 15322353
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed