Direct Evidence of Interaction of a Green Tea Polyphenol, Epigallocatechin Gallate, with Lipid Bilayers by Solid-state Nuclear Magnetic Resonance

• KUMAZAWA Shigenori

  Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka

• KAJIYA Katsuko

  Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka

• NAITO Akira

  Graduate School of Engineering, Yokohama National University

• SAITÔ Hazime

  Department of Life Science, Himeji Institute of Technology

• TUZI Satoru

  Department of Life Science, Himeji Institute of Technology

• TANIO Michikazu

  Department of Life Science, Himeji Institute of Technology

• SUZUKI Masayuki

  Food Research Laboratories, Mitsui Norin Company

• NANJO Fumio

  Food Research Laboratories, Mitsui Norin Company

• SUZUKI Eri

  Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka

• NAKAYAMA Tsutomu

  Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka

Abstract

The interaction of a tea catechin, epigallocatechin gallate (EGCg), with the model membrane of dimyristoylphosphatidylcholine (DMPC) was studied by solid-state ³¹P and ²H NMR. The ³¹P chemical shift anisotropy of the DMPC phosphate group decreased on addition of EGCg. The ²H NMR spectrum of [4-²H]EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These ³¹P and ²H NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 68 (8), 1743-1747, 2004

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• tea catechins
• epigallocatechin gallate
• liposome
• lipid bilayer
• solid-state NMR

Details

• CRID: 1390282681453006080
• NII Article ID: 10013520988
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.68.1743
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7066970
• PubMed: 15322359
• Web Site: http://id.ndl.go.jp/bib/7066970
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7066970
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.68.1743
• Web Site: https://search.jamas.or.jp/link/ui/2006162392
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed