Oxysterol Regulation of Estrogen Receptor α-Mediated Gene Expression in a Transcriptional Activation Assay System Using HeLa Cells

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Author(s)

    • Sato Hiroyoshi SATO Hiroyoshi
    • Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University
    • Nishida Satoko NISHIDA Satoko
    • Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University
    • SATO Masao
    • Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University
    • IKEDA Ikuo
    • Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University
    • IMAIZUMI Katsumi
    • Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University

Abstract

In order to test the estrogenic activity of sterol oxidation products from cholesterol and phytosterols, an estrogen-dependent gene expression assay was performed in estrogen receptor α-stably transformed HeLa cells. The ranking of the estrogenic potency of these compounds was different: 17β-estradiol >> genistein >> β-epoxycholesterol = daidzein = cholestanetriol = 22(R)-hydroxycholesterol = 20(S)-hydroxycholesterol = sitostanetriol > campestanetriol = β-epoxysitosterol = 7β-hydroxycholesterol. These compounds were not estrogenic in estrogen receptor-negative HeLa cells.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 68(8), 1790-1793, 2004-08-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  12

Codes

  • NII Article ID (NAID)
    10013521146
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    09168451
  • NDL Article ID
    7067055
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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