Oxysterol Regulation of Estrogen Receptor .ALPHA.-Mediated Gene Expression in a Transcriptional Activation Assay System Using HeLa Cells

• SATO Hiroyoshi

  Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University

• NISHIDA Satoko

  Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University

• TOMOYORI Hiroko

  Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University

• SATO Masao

  Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University

• IKEDA Ikuo

  Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University

• IMAIZUMI Katsumi

  Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University

Bibliographic Information

Other Title

• Oxysterol Regulation of Estrogen Receptor α-Mediated Gene Expression in a Transcriptional Activation Assay System Using HeLa Cells
• Oxysterol Regulation of Estrogen Receptor アルファ Mediated Gene Expression in a Transcriptional Activation Assay System Using HeLa Cells

Abstract

In order to test the estrogenic activity of sterol oxidation products from cholesterol and phytosterols, an estrogen-dependent gene expression assay was performed in estrogen receptor α-stably transformed HeLa cells. The ranking of the estrogenic potency of these compounds was different: 17β-estradiol >> genistein >> β-epoxycholesterol = daidzein = cholestanetriol = 22(R)-hydroxycholesterol = 20(S)-hydroxycholesterol = sitostanetriol > campestanetriol = β-epoxysitosterol = 7β-hydroxycholesterol. These compounds were not estrogenic in estrogen receptor-negative HeLa cells.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 68 (8), 1790-1793, 2004

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• oxysterols
• estrogen receptor
• cholesterol
• phytosterol
• phytoestrogen

Details

• CRID: 1390001206476181248
• NII Article ID: 10013521146
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.68.1790
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7067055
• PubMed: 15322366
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7067055
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.68.1790
• Web Site: https://search.jamas.or.jp/link/ui/2006162394
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed