Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene of<i>Lotus japonicus</i>

  • KINOSHITA Natsuko
    Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University
  • OOKI Yasuhiro
    Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University
  • DEGUCHI Yuichi
    Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University
  • CHECHETKA Svetlana A.
    Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University
  • KOUCHI Hiroshi
    National Institute of Agrobiological Sciences
  • UMEHARA Yosuke
    National Institute of Agrobiological Sciences
  • IZUI Katsura
    Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University
  • HATA Shingo
    Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University

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  • Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene of Lotus japonicus

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We isolated a cDNA encoding mitogen-activated protein kinase kinase kinase alpha, designated LjM3Kalpha, from Lotus japonicus, a model legume. The gene was expressed constitutively in roots, root nodules, and shoots. We also identified a novel nodulin gene, LjNUF, that shows specific expression in nodules. LjNUF resembles the C-terminal half of a hypothetical protein (pir//D85436), the N-terminal half of which is similar to a portion of mitogen-activated protein kinase kinase kinase gamma. Although LjNUF was predicted to be a secreted protein, its function remains to be clarified.

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