Proteomic Studies of Lipopolysaccharide-induced Polypeptides in the Silkworm, Bombyx mori

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  • WANG Yongqiang
    Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University Zhejiang Academy of Agricultural Sciences
  • ZHANG Pingbo
    Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
  • FUJII Hiroshi
    Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
  • BANNO Yutaka
    Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
  • YAMAMOTO Kohji
    Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
  • ASO Yoichi
    Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University

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  • Proteomic Studies of Lipopolysaccharide-induced Polypeptides in the Silkworm,<i>Bombyx mori</i>

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Abstract

Silkworm larvae at the 5th instar were injected with lipopolysaccharide from Escherichia coli and inducible polypeptides were examined within a pI range of 3–10 and a size range of 14–97 kDa by proteomics, including peptide mass fingerprinting. No polypeptides were induced in the midgut. FB1 and H1–4 polypeptides were significantly induced in fat body and hemolymph, respectively. FB1 and H1 were estimated to be antitrypsin and serpin-2 proteinase inhibitors respectively. H2 and H3 were novel polypeptides. H4 was estimated to be attacin antibacterial polypeptide with high coverage of sequence. The amounts of all the induced polypeptides decreased at 48 h after the injection.

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