Anthocyanin galactosyltransferase from Aralia cordata, cDNA cloning and characterization

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The UDP-galactose: anthocyanin 3-O-galactosyltransferase (ACGaT) cDNA was cloned from cell-suspension cultures of Aralia cordata Thunb. (Araliaceae). The cDNA was isolated from a cDNA library using a galactosyltransferase cDNA from Vigna mungo. The cDNA contained an open reading frame encoding 452 amino acids with a calculated molecular mass of 50.0 kDa. The deduced amino acid sequence of ACGaT cDNA included a plant secondary product glycosyltransferase signature sequence and was similar to the flavonoid 3-O-glycosyltransferases from petunia and grape. The enzymatic activity of the recombinant ACGaT expressed in Escherichia coli was detected toward anthocyanins and flavonols as an aglycon and UDP-galactose as a sugar donor. The reaction product for quercetin and UDP-galactose catalyzed by the recombinant ACGaT was identified as quercetin 3-O-β-galactoside using nuclear magnetic resonance spectroscopy.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 21(3), 191-195, 2004-09-01

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10013522761
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    7066683
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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