Anthocyanin galactosyltransferase from Aralia cordata, cDNA cloning and characterization
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- NAGASHIMA Shigeyuki
- School of Pharmaceutical Sciences, Kitasato University
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- OKAMOTO Aya
- School of Pharmaceutical Sciences, Kitasato University
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- SUZUKI Hideyuki
- School of Pharmaceutical Sciences, Kitasato University
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- ASADA Yoshihisa
- School of Pharmaceutical Sciences, Kitasato University
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- KONDO Toshiya
- School of Pharmaceutical Sciences, Kitasato University
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- YOSHIKAWA Takafumi
- School of Pharmaceutical Sciences, Kitasato University
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Abstract
The UDP-galactose: anthocyanin 3-O-galactosyltransferase (ACGaT) cDNA was cloned from cell-suspension cultures of Aralia cordata Thunb. (Araliaceae). The cDNA was isolated from a cDNA library using a galactosyltransferase cDNA from Vigna mungo. The cDNA contained an open reading frame encoding 452 amino acids with a calculated molecular mass of 50.0 kDa. The deduced amino acid sequence of ACGaT cDNA included a plant secondary product glycosyltransferase signature sequence and was similar to the flavonoid 3-O-glycosyltransferases from petunia and grape. The enzymatic activity of the recombinant ACGaT expressed in Escherichia coli was detected toward anthocyanins and flavonols as an aglycon and UDP-galactose as a sugar donor. The reaction product for quercetin and UDP-galactose catalyzed by the recombinant ACGaT was identified as quercetin 3-O-β-galactoside using nuclear magnetic resonance spectroscopy.
Journal
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- Plant Biotechnology
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Plant Biotechnology 21 (3), 191-195, 2004
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679304000256
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- NII Article ID
- 10013522761
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2cXptVKhtbY%3D
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 7066683
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed