Occurrence of Two Forms of γ-Glutamyltransferases in Radish Plant

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著者

    • KOIZUMI Yukio
    • Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
    • SEKIYA Jiro
    • Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

抄録

In radish (Raphanus sativus L.) cotyledons, γ-glutamyltransferase (GGT, EC 2.3.2.2) activity was detected in both fractions extracted with grinding buffer alone (low ionic strength buffer) and the same buffer supplemented with 1 M NaCl (high ionic strength buffer). The former was referred to as soluble GGT and the latter, as bound GGT. Bound GGT was not solubilized by 1% Triton X-100. The activities of both soluble and bound GGTs were inhibited by acivicin, an inhibitor of mammalian and Escherichia coli GGTs. The activity of GGT extracted with the high ionic strength buffer disappeared when protoplasts were prepared, indicating the localization of bound GGT to cell walls. These results strongly indicate the occurrence of two forms of GGTs, soluble and bound ones. The two forms of GGTs were also found in Arabidopsis thaliana, cabbage, pea, soybean, maize and rice among the eight plants tested, in addition to radish.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 21(3), 243-246, 2004-09-01

    日本植物細胞分子生物学会

参考文献:  24件中 1-24件 を表示

被引用文献:  4件中 1-4件 を表示

各種コード

  • NII論文ID(NAID)
    10013522948
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    7066761
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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