Deoxyribonucleic Acid Interaction with Silanized Surfaces

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著者

    • Yoda Sawako Yoda Sawako
    • Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta Midori-ku, Yokohama 226-8501, Japan
    • Han Seung Pil Han Seung Pil
    • Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta Midori-ku, Yokohama 226-8501, Japan
    • Kwak Kwang Joo
    • Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta Midori-ku, Yokohama 226-8501, Japan
    • Fujihira Masamichi
    • Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta Midori-ku, Yokohama 226-8501, Japan

抄録

We demonstrate here the observation of the interaction between DNA and silanized cover glass surfaces using a fluorescence microscope and an atomic force microscope (AFM). Two-component surface modification with one alkylsilane mixed with another silane derivative containing amino end groups enhanced the adsorption of negatively charged DNA molecules due to the electrostatic interaction with positive charges of protonated amino end groups. We investigated the electrostatic interaction of the DNA molecules with the mixed silanized surfaces as a function of the concentration of the amino end groups. In addition, interaction on molecular scales was demonstrated by a newly developed double combing method.

収録刊行物

  • Japanese journal of applied physics. Pt. 1, Regular papers & short notes

    Japanese journal of applied physics. Pt. 1, Regular papers & short notes 43(9A), 6297-6302, 2004-09-15

    Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physics

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各種コード

  • NII論文ID(NAID)
    10013574227
  • NII書誌ID(NCID)
    AA10457675
  • 本文言語コード
    EN
  • 資料種別
    ART
  • 雑誌種別
    大学紀要
  • ISSN
    0021-4922
  • NDL 記事登録ID
    7079954
  • NDL 雑誌分類
    ZM35(科学技術--物理学)
  • NDL 請求記号
    Z53-A375
  • データ提供元
    CJP書誌  NDL  J-STAGE  JSAP 
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