Geobacillus sp.AT1株培養液添加による下水汚泥からのバイオガス生産促進効果 Enhancement of Biogas Production from Sewage Sludge with the Addition of Geobacillus sp. Strain AT1 Culture

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We investigated the enhancement of biogas production from anaerobic digestion of sewage sludge with the addition of an aerobic thermophilic bacterial culture of <I>Geobacillus</I> sp. strain AT1. Biogas production was significantly enhanced by the 2 ml addition of stationary-growth-phase culture (24h) of strain AT1 to 38 ml methanogenic sludge. Strain AT1 grew aerobically in F-JX synthetic medium, and optimum growth occurred at pH 7.0 and 65°C with shaking at 140 rpm. The optimum temperature for protease activity in the strain AT1 culture was around 65°C in the presence of 2 mM CaCl<SUB>2</SUB>, where relative protease activity reached 210% compared with that at 35°C without CaCl<SUB>2</SUB>. Protease activity was stable from 35 to 65°C, but rapidly decreased within 30 min incubation at 85°C. A protease inhibitor mixture (AEBSE, bestatin, E-64, EDTA, and pepstatin) inhibited the biogas enhancement by the strain AT1 culture. Methane production was enhanced by 2.1 times with the addition of the strain AT1 culture compared with the addition of F-JX medium, and was enhanced by 2.8 times with the additions of authentic protease and serine protease S. These results suggest that the enhancement of biogas production from anaerobic digestion of sewage sludge was caused by the protease activity of aerobic thermophilic <I>Geobacillus</I> sp. strain AT1.

収録刊行物

  • 日本水処理生物学会誌 = Journal [of] Japan Biological Society of Water and Waste

    日本水処理生物学会誌 = Journal [of] Japan Biological Society of Water and Waste 40(3), 97-104, 2004-09-15

    日本水処理生物学会

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各種コード

  • NII論文ID(NAID)
    10013592865
  • NII書誌ID(NCID)
    AN00351126
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106758
  • NDL 記事登録ID
    7096381
  • NDL 雑誌分類
    ZM24(科学技術--科学技術一般--用水・廃水)
  • NDL 請求記号
    Z14-557
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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