Lipid Analyses for the Management of Vascular Diseases

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  • Okada Masahiko
    Division of Preventive Medicine, Graduate School of Medical and Dental Sciences, Niigata University
  • Matsuto Takayuki
    Division of Preventive Medicine, Graduate School of Medical and Dental Sciences, Niigata University
  • Miida Takashi
    Division of Preventive Medicine, Graduate School of Medical and Dental Sciences, Niigata University
  • Obayashi Konen
    Division of Preventive Medicine, Graduate School of Medical and Dental Sciences, Niigata University
  • Zhu Ying
    Division of Preventive Medicine, Graduate School of Medical and Dental Sciences, Niigata University
  • Fueki Yuriko
    Division of Preventive Medicine, Graduate School of Medical and Dental Sciences, Niigata University

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Abstract

Despite a long history of studies on lipid abnormalities, numerous problems in laboratory technologies and techniques remain unresolved. One of the most commonly tested molecules is low-density lipoprotein (LDL) cholesterol, and a homogenous assay technique for measurement of this molecule has recently been introduced. Although the method represents remarkable technological breakthroughs with great potential for improving lipoprotein analysis, some discrepancies exist among assay protocols. Even for direct measurement of high-density lipoprotein (HDL) cholesterol, which has widely been accepted, there are still large discrepancies among data obtained by different protocols. Oxidatively modified LDL is an independent factor that is considered to directly contribute to the pathogenesis of early atherosclerosis. Lipid peroxidation products, surface charge, and spectrophotometric patterns are all applicable to the evaluation of in vitro oxidation. Only enzyme-linked immunosorbent assays using monoclonal antibodies have a potential for clinical use, but such methods are not yet standardized. There is also increasing evidence for the presence of anti-oxidized LDL autoantibodies in human sera, but the diagnostic utility remains controversial. In addition, small dense LDL has recently attracted much attention as an independent risk factor. Although this is a potential target of oxidization, a robust and simple analytical method does not yet exist. This review presents the current state of laboratory technologies for testing lipid abnormalities.

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