酸素電極を用いたクロノアンペロメトリーによる一般細菌数の簡易推定手法の検討 A Simple Estimation Method for Heterotrophic Plate Count by Chronoamperometry with a Dissolved Oxygen Electrode

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著者

    • 笠原 伸介 KASAHARA Shinsuke
    • 大阪工業大学工学部都市デザイン工学科 Department of Civil Engineering and Urban Design, Faculty of Engineering, Osaka Institute of Technology
    • 石川 宗孝 ISHIKAWA Munetaka
    • 大阪工業大学工学部都市デザイン工学科 Department of Civil Engineering and Urban Design, Faculty of Engineering, Osaka Institute of Technology

抄録

Applicability of a new simple method for estimating heterotrophic plate count (HPC) in environmental samples was investigated. In this method, 0.1-1.0 ml of sample is incubated in a cell equipped with a dissolved oxygen (DO) electrode. The HPC in the sample is estimated from the time required until the DO is depleted (detection time). This method was applied to a pure culture sample and environmental samples to evaluate the validity and reliability of this method.The results indicated that (1) the HPC estimated by this method was in agreement with that determined by the plate count method in environmental samples (r = 0.85), (2) 95 % confidence interval of detection time of this method was shorter than the generation time of bacteria in the samples with HPCs higher than 102-103 CFU·ml-1, (3) In the case in which the same amounts of heterotrophic bacteria existed in the sample cell, the detection time (mean value and 95 % confidence interval) for bacteria captured on a polycarbonate membrane filter (0.2 μ m) was comparable with that for bacteria suspended in the sample cell.Thus, it could be concluded that this simple method could more rapidly estimate the HPC in environmental samples than the conventional plate count method with comparable accuracy and could be sufficiently applied to low-HPC samples.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment

    水環境学会誌 = Journal of Japan Society on Water Environment 27(10), 651-657, 2004-10-10

    公益社団法人 日本水環境学会

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各種コード

  • NII論文ID(NAID)
    10013650532
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    7118329
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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