Application of Difference NOE-pumping NMR Technique and Cold-Spray Ionization Mass Spectrometry to Identify a Ligand Binding with a Protein Receptor
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- SEKI Hiroko
- Chemical Analysis Center, Chiba University CREST, Japan Science and Technology Agency (JST)
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- SEI Yoshihisa
- Chemical Analysis Center, Chiba University CREST, Japan Science and Technology Agency (JST)
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- SHIKII Kazuaki
- Chemical Analysis Center, Chiba University Analysis Research Department, Chemical Research Laboratories, Nissan Chemical Industries Ltd.
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- SHIMOTAKAHARA Sakurako
- School of Pharmacy, Tokyo University of Pharmacy and Life Science
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- UTSUMI Hiroaki
- NM Application & Research Group, Application & Research Center, Analytical Instruments Division, JEOL Ltd.
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- YAMAGUCHI Kentaro
- Chemical Analysis Center, Chiba University CREST, Japan Science and Technology Agency (JST)
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- TASHIRO Mitsuru
- Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
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抄録
A difference diffusion-based NMR technique and cold-spray ionization mass spectrometry were employed as a solution-based approach for identifying a ligand binding with a protein receptor. The difference diffusion-based NMR technique, called difference NOE-pumping, can directly detect the ligand interacting with a protein receptor. This technique uses a simple pulse sequence and the diffusion filter can easily be optimized. The cold-spray ionization mass spectrometry (CSI-MS), a variant of electrospray ionization mass spectrometry (ESI-MS) operating at low temperature, has been applied to detect the ligand-receptor complex. The efficiency of these techniques for identifying binding ligands is demonstrated with the human serum albumin (HSA)-drug system.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 20 (10), 1467-1470, 2004
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679233009664
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- NII論文ID
- 10013657686
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXovVektrk%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 7111488
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- PubMed
- 15524205
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可