<i>Cytochrome P450</i> gene expression levels in peripheral blood mononuclear cells in comparison with the liver
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<jats:p>Cytochromes P450 (CYPs) compose a superfamily of similar proteins involved in detoxification and elimination, as well as activation of a wide variety of compounds. Most CYP family members are localized in the liver. In order to assess whether peripheral blood leukocytes (PBL) are available as a surrogate for the determination of <jats:italic>CYP</jats:italic> gene expression levels in the liver, we compared <jats:italic>CYP</jats:italic> gene expression levels in PBL with those in liver tissues from patients with hepatocellular carcinoma (HCC). We measured <jats:italic>CYP1A1, 1A2, 1B1, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1, 2F1, 2J2, 3A4, 3A5, 3A7, 4A11, 4B1</jats:italic> and <jats:italic>CYP27</jats:italic> gene expressions in PBL and in the liver by real‐time reverse‐transcription (RT)‐PCR. We could detect expression of <jats:italic>CYP1A1, 1A2, P1B1, 2A6, 2B6</jats:italic> and <jats:italic>2E1</jats:italic> genes in PBL and all the genes except for <jats:italic>CYP2F1</jats:italic> in the liver. Although gene expression levels within each subfamily were closely correlated within PBL and within the liver, a clear correlation of gene expression levels between PBL and liver tissues was found only for <jats:italic>CYP4B1</jats:italic>. Although inter‐individual variation of the expression level of each <jats:italic>CYP</jats:italic> gene was wide, the induced level was proportional to the basal expression level. Therefore, monitoring of <jats:italic>CYP</jats:italic> gene expression levels in PBL, especially those of <jats:italic>CYP4B1</jats:italic>, could be available as a biomarker for monitoring of exposure to environmental pollutants and assessing the associated risk. Compared with non‐tumor tissue, HCC tissues tended to show overexpression of multiple <jats:italic>CYP</jats:italic> genes, indicating that individualized selection and more effective administration of chemotherapeutic agents could perhaps be based on the pattern of <jats:italic>CYP</jats:italic> overexpression.</jats:p>
収録刊行物
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- Cancer Science
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Cancer Science 95 (6), 520-529, 2004-06
Wiley
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詳細情報 詳細情報について
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- CRID
- 1361418520887860736
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- NII論文ID
- 10013723642
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- NII書誌ID
- AA11808050
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- ISSN
- 13497006
- 13479032
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