An Attempt to Identify Bacillus cereus by PCR

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Author(s)

    • MIYAMOTO TAKAHISA
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
    • SASAKI CHIE
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
    • SADAKARI KIYOKO
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
    • HONJOH KEN-ICHI
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
    • IIO MASAYOSHI
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University

Abstract

The DNA band patterns generated by PCR using Primer 4 and template DNAs from various<I>Bacillus</I> strains were compared. Sequence analysis of the 3.2 kb PCR product detected only in <I>B. cereus</I> revealed that the DNA sequence from base 11 to 2962 of the DNA seemed to contain a sequence specific to <I>B. cereus</I>. The primers BCF and BCR that amplify the region between base 1168 and 2320 of the 3.2 kb DNA generated the 1153 by PCR product with DNAs from <I>B. cereus</I> type strain JCM 2152, 3 cereulide-positive <I>B. cereus</I> strains and 20 strains among 49 <I>B. cereus</I> isolated from dairy products and processing factories. The same band was not amplified with those from 5 strains of <I>B. thuringiensis</I>, other 11 <I>Bacillus</I> species and 12 different bacteria which were not <I>Bacillus</I>.

Journal

  • Biocontrol Science

    Biocontrol Science 9(3), 69-75, 2004-09-20

    The Society for Antibacterial and Antifungal Agents, Japan

References:  31

Codes

  • NII Article ID (NAID)
    10013724702
  • NII NACSIS-CAT ID (NCID)
    AA11169621
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    13424815
  • Data Source
    CJP  J-STAGE 
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