高血圧自然発症ラットを用いたα-トコフェロールとイワシタンパク質由来ペプチド併用による血圧調節作用の解析  [in Japanese] Synergic Effects of α-Tocopherol and Peptide Derived from Sardine Protein Hydrolysates on Blood Pressure in Spontaneously Hypertensive Rats  [in Japanese]

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Abstract

高血圧自然発症ラット(SHR)を用いてα-トコフェロール(α-Toc)連続投与下でイワシタンパク質由来ペプチド(Y-2)を併用した時の降圧作用を検討した.<br>ラットをα-Toc非投与群,α-Toc低用量(α-Toc32mg/kg体重)群,α-Toc高用量(α-Toc160mg/kg体重)群の3群に分け,経口投与を42日間継続し,尾動脈圧(収縮期血圧:SBP)を測定した結果,α-Toc投与群では非投与群と比較して投与期間中を通じてSBPの低下傾向が観察された.<br>それぞれの群をさらに2群に分け,Y-2非投与群あるいはY-2投与(100mg/kg体重)群の計6群とし,Y-2単回投与後,経時的にSBPを測定した.Y-2の単回投与によりα-Tocの用量にかかわらず全ての群において有意に降圧作用が確認され,Y-2による降圧の程度にはα-Tocへの用量依存性が認められた.α-Toc連続投与下でのY-2単回投与6時間後では,血漿中のα-Toc量はα-Toc投与群で有意に増加し,α-Toc高用量+Y-2投与群ではα-Toc非投与+Y-2非投与群と比較してTAS,LPOなどの抗酸化指標の有意な改善が見られた.ACE阻害作用は主に心臓において有意な低下が見られた.以上の結果からα-Tocの抗酸化作用とY-2のACE阻害作用が協働して,降圧効果が増強された可能性が示された.

We examined the antihypertensive effects of α-tocopherol (α-Toc) and the peptide derived from sardine protein hydrolysates (Y-2) on spontaneously hypertensive rats (SHRs). In repeated oral administration of α-Toc at the low dose (32mg/kg/day) or high dose (160mg/kg/day) for 42 days, the systolic blood pressure (SBP) of SHR was declined compared to that of control. Single oral administration of Y-2 after repeated oral administration of α-Toc significantly lowered the SBP in SHR. These effects lasted for 6-8h after administration of Y-2. The hypotensive effect of Y-2 was reinforced by repeated oral administration of α-Toc dependently with its dose. At the end of the experiment, experimental animals were killed six hours after single oral administration of Y-2. In rats administrated with α-Toc, the plasma α-Toc concentration was significantly higher than that of rats administrated with the vehicle. In rats administrated with a high dose of α-Toc and Y-2, antioxidant parameters, plasma lipid peroxide and total antioxidant status were significantly improved and the angiotensin I-converting enzyme (ACE) activity of the heart was significantly lower than that of rats administrated with the vehicle. These results suggest that the hypotensive effect of α-Toc and Y-2 is correlated with the ACE inhibitory activity of Y-2 and antioxidant activity of α-Toc. The combination of α-Toc and Y-2 will be beneficial for improving blood pressure in hypertension.

Journal

  • Nippon Shokuhin Kagaku Kogaku Kaishi

    Nippon Shokuhin Kagaku Kogaku Kaishi 51(10), 546-553, 2004-10-15

    Japanese Society for Food Science and Technology

References:  27

Codes

  • NII Article ID (NAID)
    10013724878
  • NII NACSIS-CAT ID (NCID)
    AN10467499
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    1341027X
  • NDL Article ID
    7123995
  • NDL Source Classification
    ZP34(科学技術--食品工学・工業)
  • NDL Call No.
    Z17-126
  • Data Source
    CJP  NDL  J-STAGE 
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