Discrimination of Isomeric Fragment Ions Observed in Tandem Mass Spectra of Biantennary Oligosaccharides by Use of Selective Isotope Labeling

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抄録

We report herein the first mass spectrometric analyses of isotopomers of a pyridylamino (PA)-derivatized biantennary oligosaccharide, in which one of two non-reducing terminal galactose residues is selectively enriched in <sup>13</sup>C by an enzymatic reaction. By use of these isotopomeric PA-oligosaccharides, individual fragment ions observed in tandem mass spectra of MALDI-QIT-TOF-MS were unambiguously assigned. It was shown that the oligosaccharide ion that lacks the <i>N</i>-acetyllactosamine previously linked to the Manα1 →6 antenna dominates its isomeric counterpart ion. We propose that tandem mass spectrometric analyses of oligosaccharides isotopically labeled at a selected antenna would provide us with the basis of mechanisms of formation of glycosidic linkage fragment ions.

収録刊行物

  • 質量分析 = Mass spectroscopy

    質量分析 = Mass spectroscopy 52(5), 284-288, 2004-10-01

    一般社団法人 日本質量分析学会

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各種コード

  • NII論文ID(NAID)
    10013736894
  • NII書誌ID(NCID)
    AN0010555X
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13408097
  • NDL 記事登録ID
    7132551
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z17-213
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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