Development of a New Experimental System for Monitoring Biomembrane Reactions: Combination of Laser Spectroscopic Techniques and Biomembrane Models Formed at an Oil/Water Interface
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- MORISAKU Toshinori
- Department of Advanced Materials Science, Graduate School of Frontier Sciences, The University of Tokyo
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- YUI Hiroharu
- Department of Advanced Materials Science, Graduate School of Frontier Sciences, The University of Tokyo Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)
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- SAWADA Tsuguo
- Department of Advanced Materials Science, Graduate School of Frontier Sciences, The University of Tokyo Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)
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抄録
We present a new experimental system to observe reactions in biomembranes by combining laser spectroscopic techniques with phospholipid monolayers formed at oil/water interfaces. The system can monitor reactions through changes in interfacial tension at oil/water interfaces induced by the reactions under non-destructive and non-contact conditions. In addition, oil/water interfaces with defined areas can define the composition of different kinds of phospholipids. Furthermore, the system allows using, as an oil phase, alkanes whose number of carbon atoms is close to the number of the alkyl chains of phospholipids in biomembranes (C ≥ 16). We demonstrated the hydrolysis reaction in DPPC (dipalmitoyl phosphatidylcholine)/DPPS (dipalmitoyl phosphatidylserine)-mixed monolayers by phospholipase A2 by using the system.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 20 (11), 1605-1608, 2004
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679234821120
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- NII論文ID
- 130004440865
- 10013773893
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXhtVWgs7%2FN
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 7146497
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- PubMed
- 15566157
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可