河川水の酵母 Two-Hybrid 試験における女性ホルモン活性物質の濃縮回収方法と試験阻害物質の除去方法の開発 Concentration and Inhibitor Removal Methods for Determination of Estrogenic Activity in River Water using Yeast Two-Hybrid Test

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著者

    • 細川 将洋 HOSOKAWA Masahiro
    • 横浜国立大学大学院環境情報学府/研究院 Graduate School of Environment and Information Sciences, Yokohama National University
    • 劉 鋭 LIU Rui
    • 横浜国立大学ベンチャー・ビジネス・ラボラトリー Venture Business Laboratory, Yokohama National University
    • 久保 隆 KUBO Takashi
    • 横浜国立大学大学院環境情報学府/研究院 Graduate School of Environment and Information Sciences, Yokohama National University
    • 浦野 紘平 URANO Kohei
    • 横浜国立大学大学院環境情報学府/研究院 Graduate School of Environment and Information Sciences, Yokohama National University

抄録

To apply the yeast two-hybrid test for the evaluation of river water, an effective method of concentrating estrogenic substances and a method of removing inhibitors were developed. When 250 ml of river water was supplied to a Sep-Pak Plus PS-2 cartridge and then 10 ml of acetone was used for desorption, typical estrogenic substances were recovered by as much as 93-101% and were concentrated quickly to 500-fold. Moreover, when the concentrated water sample was supplied to a column containing 1.5g of silica gel and was eluted with 8 ml of 30% acetone in toluene, inhibitors of estrogenic activity were removed. The validity of these methods was confirmed using actual river water samples obtained from 19 sites of the Sagami River and 7 sites of the Ayase River.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment

    水環境学会誌 = Journal of Japan Society on Water Environment 27(11), 679-684, 2004-11-10

    公益社団法人 日本水環境学会

参考文献:  18件中 1-18件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10013786651
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    7149089
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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