Expression of mRNAs for Growth Factor Receptors during Development In Vitro of Porcine Ova Matured and Fertilized In Vitro.

  • Yoshida Yumiko
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
  • Yoshida Mitsutoshi
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

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  • ブタ体外成熟・体外受精卵の体外発生過程における成長因子レセプター遺伝子発現
  • Expression of m RNA s for Growth Factor

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Abstract

We analyzed the expression of mRNAs for growth factor receptors with receptor tyrosine kinases [ErbB3 (a member of the EGF-R subfamily), insulin-like growth factor-I receptor (IGF-I-R), basic fibroblast growth factor receptor (bFGF-R) and platelet-derived growth factor receptor α (PDGF-Rα)] by reverse transcription- polymerase chain reaction during in vitro development (10 to 168 h of in vitro insemination: zygotes to blastocysts) of porcine ova matured and fertilized in vitro. Transcripts for IGF-I-R, bFGF-R and PDGF-Rα were detectable in ova at all stages of in vitro development. Transcripts for ErbB3 were present in embryos from the late pronucleate stage onward. The results provide evidence that several growth factor receptor mRNAs are present in porcine ova during development in vitro.<br>

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