ブタ卵胞卵子の第一減数分裂中期（M I）から第二減数分裂中期（M II）への減数分裂進行における卵丘細胞内PI 3-kinaseの役割

• 島田 昌之

  広島大学生物生産学部

• Anas Mohamed-Kheir Idris

  Graduate School for International Development and Cooperation, Hiroshima University

• 寺田 隆登

  広島大学生物生産学部

書誌事項

タイトル別名

• Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic Progression from M I to M II Stage in Porcine Follicular Oocytes.
• Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic Progression from M1 to M2 Stage in Porcine Follicular Oocytes
• Phosphatidylinositol 3-Kinase in Cumulu

抄録

Porcine cumulus oocyte complexes (COCs) were cultured in an inhibitor free-medium for 24 h and then some of them were denuded. The COCs and denuded oocytes (DOs) were further cultured for 24 h in the presence of Phosphatidylinositol 3-kinase (PI 3-kinase) specific inhibitors, wortmannin and LY294002. In COCs and DOs, wortmannin (10^-8, 10^-7 M) and LY294002 (5.0 × 10^-5 M) significantly increased the proportion of oocytes arrested at M I and decreased that of oocytes reaching M II. Nevertheless, in DOs the differences between in the presence of the inhibitors and the control in the proportion of oocytes reaching M II substantially diminished as compared to those in the case of COCs. Cumulus cell expansion was significantly suppressed by wortmannin and LY294002. It is concluded that PI 3-kinase in cumulus cells plays a regulatory role in meiotic progression beyond M I and cumulus cell expansion in porcine COCs.<br>

収録刊行物

• 日本哺乳動物卵子学会誌

  日本哺乳動物卵子学会誌 15 (1), 68-76, 1998

  日本卵子学会

キーワード

• PI 3-kinase
• ブタ卵子
• 減数分裂
• ワルトマニン
• LY294002

詳細情報

• CRID: 1390001204338815872
• NII論文ID: 10013835156
• NII書誌ID: AN10548943
• DOI: 10.1274/jmor.15.68
• ISSN: 13475878
• ISSN: 13417738
• NDL書誌ID: 4448318
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030591996
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4448318
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可