HUMAN CELL LINE IDENTIFICATION BASED ON D1S80 AND HLA DQα GENOTYPING BY PCR-AMPLIFICATION OF ALLELES

• TAKAHASHI Masanori

  Department of Legal Medicine, Dokkyo University School of Medicine

• KATO Yukie

  Department of Legal Medicine, Dokkyo University School of Medicine

• MANAKA Ken-ichi

  Research Center of Tissue Culture, Dokkyo University School of Medicine

• MUKOYAMA Harutaka

  Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association

• MIYAKAWA George

  Department of Legal Medicine, Dokkyo University School of Medicine

• KAMIYAMA Shigetaro

  Department of Legal Medicine, Dokkyo University School of Medicine

Abstract

The identification of the human cultured cell lines was performed by the method of the DNA-polymorphism typing assay based on a polymerase chain reaction (PCR). We have examined eight human cell lines given from Japanese Cancer Research Resources Bank (A-549, HeLa, IMR-32, HT-1080, RD, PA-1, PC-3 and RERF-LC-MS). Each locus of D1S80 and HLA DQa was amplified by PCR. Alleles of the D1S80 were observed as silver-stained bands after electrophoresis on a polyacrylamide gel, and the HLA DQa product was screened onto strips dotted the sequence-specific DNA probes by a reverse dot-blot hybridization. Each cell line was distinct independently in the types of D1S80-VNTR and HLA DQα sequences.<br>The cumulative power of discrimination (PD) for D1S80 and HLA DQα types was calculated to be 0.996-0.998 from the racial population study. The superiority of this method to the fingerprinting one would be its reproducible results and shortening of process time. The system used here for the detection of D1S80 and HLA DQα alleles would be one of the standards to establish cell libraries.

Journal

• TISSUE CULTURE RESEARCH COMMUNICATIONS

  TISSUE CULTURE RESEARCH COMMUNICATIONS 14 (2), 103-107, 1995

  The Japanese Tissue Culture Association

Keywords

• D1S80
• HLA-DQα
• Polymorphism
• PCR
• Cell-identification

Details

• CRID: 1390001205466466944
• NII Article ID: 10013840025
• NII Book ID: AA11048252
• DOI: 10.11418/jtca1981.14.2_103
• ISSN: 09123636
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed