Immunolocalization of CD44 and Heparan Sulfate Chains on the Stratum Intermedium and Papillary Layer in the Rat Enamel Organ

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著者

    • KIMURA Shin
    • 新潟大学歯学部 Department of Pathology, Niigata University School of Dentistry
    • KENMOTSU Shin-ichi
    • 新潟大学歯学部口腔解剖学第一講座 Department of Oral Anatomy, Niigata University School of Dentistry
    • SAKAI Hideo
    • 新潟大学歯学部口腔解剖学第一講座 Department of Oral Anatomy, Niigata University School of Dentistry
    • SAKU Takashi
    • 新潟大学歯学部 Department of Pathology, Niigata University School of Dentistry
    • OZAWA Hidehiro
    • 新潟大学歯学部口腔解剖学第一講座 Department of Oral Anatomy, Niigata University School of Dentistry

抄録

We studied the immunohistochemical localization of CD44 and heparan sulfate (HS) chains in rat enamel organ by confocal laser scanning microscopy and transmission electron microscopy. We also investigated the binding sites of basic fibroblast growth factor (bFGF), one of the heparin-binding growth factors (HBGF), on Microslicer-sections to clarify its role in the cell-cell interaction of HS.<br>At the differentiation stage of ameloblasts, weak immunoreactivity for CD44 was detected on the plasma membrane of the inner enamel epithelium, external enamel epithelium and the cells adjacent to the inner enamel epithelium. In accordance with the differentiation of preameloblasts into secretory ameloblasts, this immunoreactivity increased in stratum intermedium cells. At the secretory stage, stratum intermedium cells showed the most intense immunoreactivity in the enamel organ. At the maturation stage, strong immunoreactiviry was seen on papillary layer cells. On the other hand, the lateral plasma membrane of ruffleended (RA) and smooth-ended ameloblast (SA) showed weak reactivity. No immunoreactivity was detected on the ruffled border of RA and the distal plasma membrane of SA. Immunolocalization of HS chains was similar to that of CD44. The binding activity of bFGF was also intense on stratum intermedium cells and papillary layer cells.<br>These findings suggest that: 1) stratum intermedium cells and papillary layer cells express CD44 and HS chains in accordance with their differentiation; 2) HS chains on the plasma membrane of these cells may regulate calcium transport by their negative charge; and 3) HS chains on the stratum intermedium and papillary layer may play an important role in the differentiation and activity of ameloblasts by preserving HBGF.

収録刊行物

  • Archives of histology and cytology

    Archives of histology and cytology 58(3), 323-334, 1995-08-01

    国際組織細胞学会

参考文献:  39件中 1-39件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10013861667
  • NII書誌ID(NCID)
    AA1068990X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09149465
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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