Cationic Colloidal Gold Staining of Acidic Glycoconjugates in Mouse Paneth Cells

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著者

    • 村田 長芳 GE Ying Bin
    • 鹿児島大学医学部第二解剖学教室 Department of Anatomy, Faculty of Medicine, Kagoshima University
    • YANG Dong Hua
    • 鹿児島大学医学部第二解剖学教室 Department of Anatomy, Faculty of Medicine, Kagoshima University
    • OHMORI Jun
    • 鹿児島大学医学部第二解剖学教室 Department of Anatomy, Faculty of Medicine, Kagoshima University
    • TSUYAMA Shinichiro
    • 鹿児島大学医学部第二解剖学教室 Department of Anatomy, Faculty of Medicine, Kagoshima University
    • KIM Bong Seon
    • Department of Anatomy, Pusan National University Medical College
    • KIM Jae Bong
    • Department of Anatomy, Pusan National University Medical College

抄録

The acidic glycoconjugates of mouse ileum Paneth cells were examined with the aid of light and electron microscopy, using cationic colloidal gold (CCG) as a probe. Specimens of mouse ilea were fixed in half-strength Karnovsky's fixative and embedded in Lowicryl K4M resin. Semithin and ultrathin sections were cut for examination with light and electron microscopy, respectively.<br>Examination of the sections using light microscopy revealed the positive staining of CCG at pH 1.0 and pH 2.5, which was detected at the rim of secretory granules and at the supranuclear regions of the Paneth cells. At pH 4.0, in addition to staining of the secretory granule rim, weak staining was observed in the granule core. At pH 7.2, the cytoplasm other than secretory granules exhibited positive CCG staining.<br>Examination of the sections using electron microscopy, at pH 1.0, the <i>trans</i> lamellae of the Golgi apparatus, the rim of the secretory granules, and lysosomes were labeled selectively by CCG. At pH 2.5, labeling was also discernible over the same structures in the cells. However, at this pH, the labeling intensity was stronger than that at pH 1.0, due to the dual labeling of sulfated and sialylated glycoconjugates in these structures. At pH 4.0, the Golgi apparatus, rims and cores of secretory granules and ribosomes were labeled. Lysosomes and nuclei were also positively stained. At pH 7.2, the rims of secretory granules were not stained.<br>The present results indicate that the CCG method gives good resolution and contrast when applied to staining, and therefore is useful for the specific staining of glycoconjugates such as sulfated, sialylated and phosphated glycoconjugates for light and electron microscopy.

収録刊行物

  • Archives of histology and cytology

    Archives of histology and cytology 60(2), 133-142, 1997-06-01

    International Society of Histology and Cytology

参考文献:  42件中 1-42件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10013864903
  • NII書誌ID(NCID)
    AA1068990X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09149465
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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