閉経が歯肉溝滲出液中のインターロイキン-1濃度に及ぼす影響  [in Japanese] Effect of Menopause on Interleukin-1 Concentration in Gingival Crevicular Fluids  [in Japanese]

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Author(s)

    • 夫馬 大介 FUMA Daisuke
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 稲垣 幸司 INAGAKI Koji
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 石原 裕一 ISHIHARA Yuichi
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 小出 雅則 KOIDE Masanori
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 田中 繁寿 TANAKA Shigehisa
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 伊藤 貴志 ITO Takashi
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 岩田 武久 IWATA Takehisa
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 鈴木 万里代 SUZUKI Mariyo
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 黒須 康成 KUROSU Yasunari
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 佐藤 連造 SATO Renzo
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 坂野 雅洋 SAKANO Masahiro
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 杉石 泰 SUGIISHI Tai
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University
    • 吉成 伸夫 YOSHINARI Nobuo
    • 愛知学院大学歯学部歯科保存学第三講座 Department of Periodontology, School of Dentistry, Aichi-Gakuin University

Abstract

エストロゲンの欠乏は, 閉経後骨粗鬆症に関連し, エストロゲンは, 骨吸収抑制作用があると考えられている。閉経および卵巣摘出により, 骨吸収性サイトカインの産生が増加する。そこで本研究では, 閉経後女性の歯肉溝滲出液 (gingival crevicular fluid, GCF) 中のIL-1濃度を, 閉経前女性との間で比較した。IL-1α, IL-1β, IL-1ra濃度および総IL-1/IL-1ra比 (IL-1活性度, IL-1 AI) は, 閉経前女性 (Pr群, 44.2±2.2歳) 12名の72歯, 閉経後女性 (Po群, 57.8±1.3歳) 12名の72歯, 合計144歯から測定した。IL-1α, IL-1βおよびIL-1ra濃度の測定には, サンドウィッチ酵素免疫測定法 (enzyme linked immunosorbent assay, ELISA) を用いた。GCF採取時に, 歯周病所見 (プロービングポケット深さ, クリニカルアタッチメントレベル, プロービング時の歯肉出血, 歯の動揺度, 歯肉炎指数, 歯槽骨吸収率) を診査した。歯周病所見は, 両群間に差はなく, 辺縁歯肉の炎症はコントロールされていた。Po群のIL-1α, IL-1ra濃度は, Pr群に比べ高く (<i>P</i> < 0.01), また, IL-1β濃度, IL-1AIも高かった (<i>P</i> < 0.05)。したがって, 閉経は, GCF中のIL-1産生の増加に影響することが示唆された。

Estrogen is thought to play a role in inhibiting bone resorption and reduced estrogen levels correlate with postmenopausal osteoporosis. Menopause and oophorectomy have been associated with increased production of bone resorption-inducing cytokines. The purpose of the present study was to compare the concentrations of Interleukin-1 (IL-1) in the gingival crevicular fluid (GCF) of premenopausal and postmenopausal women. IL-1 α, IL-1 β, IL-1 receptor antagonist (ra) and the total IL-1/IL-1 ra ratio (IL-1 activity index ; IL-1 AI) were measured in 144 GCF samples from 72 sites in 12 premenopausal patients (Pr group : age 44.2±2.2) and 72 sites in 12 postmenopausal patients (Po group : age 57.8±1.3). IL-1 α, IL-1 β and IL-1 ra were measured by specific non-crossreactive enzyme linked immunosorbent assays. The probing depth, clinical attachment level, bleeding on probing, tooth mobility, gingival index, and alveolar bone loss of each site were recorded at the time of GCF sampling. The marginal gingival inflammation in both groups was well controlled and the clinical inflammatory assessments of the gingival condition were indistinguishable in both groups. The concentrations of IL-1 α, IL-1 β, IL-1 ra and IL-1 AI in the Po group were significantly higher than those in the Pr group (IL-1 α 110.0±15.1 pg/m<i>l</i> ; 61.3±8.8 pg/m<i>l</i>, <i>P</i> < 0.01, IL-β 27.3±4.8 pg/m<i>l</i> ; 15.6±3.4 pg/m<i>l</i>, <i>P</i> < 0.05, IL-1 ra 77.2±13.6 ng/m<i>l</i> ; 39.9±4.3 ng/m<i>l</i>, <i>P</i> < 0.01, IL-1 AI, 27.3±4.8 pg/m<i>l</i> ; 15.6±3.4 pg/m<i>l</i>, 86951<0.05). Conclusively, the increased IL-1 α, IL-1 β, IL-1 ra concentrations and IL-1 AI in the Po group suggest that menopause does correlate with increased IL-1 cytokine in GCF.

Journal

  • Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)

    Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 46(4), 259-265, 2004-12-28

    JAPANESE SOCIETY OF PERIODONTOLOGY

References:  31

Cited by:  2

Codes

  • NII Article ID (NAID)
    10014078774
  • NII NACSIS-CAT ID (NCID)
    AN0019129X
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03850110
  • Data Source
    CJP  CJPref  J-STAGE 
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