Regeneration of transformed Ophiorrhiza pumila plants producing camptothecin

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  • Watase Ikumi
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University
  • Sudo Hiroshi
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University
  • Yamazaki Mami
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University
  • Saito Kazuki
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University

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Abstract

Camptothecin derivatives are clinically used as anti-tumor alkaloids that are currently obtained by extraction from intact plants. Seeking for the alternative sources for commercial production and for fundamental study, cell and tissue cultures have been investigated. In the present study, we developed a method for regeneration of Ophiorrhiza pumila plant from hairy roots transformed with Agrobacterium rhizogenes. The regeneration frequency was over 83%. Integration of a rol B gene from T-DNA of A. rhizogenes was confirmed by polymerase chain reaction in both of the hairy roots and the regenerated plants. The transformed plants accumulated camptothecin in amounts of 66–111% compared with that in the wild-type plants.

Journal

  • Plant Biotechnology

    Plant Biotechnology 21 (5), 337-342, 2004

    Japanese Society for Plant Biotechnology

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