Elicitor-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures: Activities of rosmarinic acid synthase and the final two cytochrome P450-catalyzed hydroxylations
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- Ogata Ayako
- Graduate School of Pharmaceutical Sciences, Nagoya City University
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- Tsuruga Ayako
- Graduate School of Pharmaceutical Sciences, Nagoya City University
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- Matsuno Michiyo
- Graduate School of Pharmaceutical Sciences, Nagoya City University
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- Mizukami Hajime
- Graduate School of Pharmaceutical Sciences, Nagoya City University
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A drastic increase in rosmarinic acid (RA) accumulation is induced in cultured cells of Lithospermum erythrorhizon after their exposure to yeast extract or methyl jasmonate. Addition of the elicitors to the cell cultures only slightly enhanced the activity of rosmarinic acid synthase, which catalyzes formation of 4-coumaroyl-4′-hydroxyphenyllactic acid (CHPL), the first reaction specific to RA biosynthesis. In contrast, the two-step cytochrome P450-catalyzed hydroxylations of CHPL to form RA were dramatically up-regulated by the elicitor treatments, indicating that these hydroxylation activities are likely to play a key regulatory role in elicitation of RA biosynthesis.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 21 (5), 393-396, 2004
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679305442816
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- NII論文ID
- 10014086467
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2MXht1Kks74%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 7200873
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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