Quantitative Analysis of Messenger RNA Expression of Lysyl Hydroxylases in Mandibular and Femoral Bone Marrow of Senescence-accelerated Mice

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Abstract

Messenger RNA analysis of bone marrow is a potential examination to diagnose the quality of maxillofacial bone. Lysyl hydroxylases (LHs) are responsible for collagen cross-linking and determine bone quality. To investigate mRNA expression patterns of LHs in bone marrow with potentially different bone quality, we quantified the mRNA expression of LH isoforms (LH1, LH2, and LH3), type I collagen (COLI), and alkaline phosphatase (ALP) in the mandibular and femoral bone marrows of three senescence-accelerated mouse (SAM) strains; the osteoporosis model SAMP6, mandibular osteoarthritis model SAMP8, and their control SAMR1. Total RNAs directly isolated from the bone marrows were used for reverse transcription and the products were then used to quantify mRNA expressions of the molecules using a real-time PCR assay. The expression levels of all the molecules varied in the mandibular and femoral bone marrows. The expression levels of COLI, ALP, and LH2 were higher in the mandibular than in the femoral bone marrow. In the mandibular bone marrow, the expression levels of COLI were significantly different among the three SAM strains. In both bone marrows, an association was observed between COLI and LH1 expressions, and between ALP and LH2 expressions. This study indicates that gene expression patterns of LH1 and LH2 in bone marrows are associated with those of developmental osteoblast markers and that the expression patterns of LH2 and the markers are different in mandibular and femoral bone marrows. Quantitative mRNA analysis of LHs in combination with developmental osteoblast markers in bone marrow may be useful to investigate their relationship with the quality of maxillofacial bone.

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