Isolation of mutants with aberrant mitochondrial morphology from Arabidopsis thaliana

• Feng Xiaoge

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Arimura Shin-ichi

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Hirano Hiro-Yuki

  Graduate School of Science, The University of Tokyo

• Sakamoto Wataru

  Research Institute for Bioresources, Okayama University

• Tsutsumi Nobuhiro

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

Abstract

To identify genes related to plant mitochondrial morphology and dynamics, novel mutants with respect to mitochondrial morphology were isolated from an ethyl methane sulphonate (EMS)-mutated population of Arabidopsis thaliana. Mitochondria were visualized by transforming Arabidopsis with a gene for a fusion protein consisting of GFP and a mitochondria-targeting pre-sequence. From 19,000 M2 populations, 17 mutants were isolated by fluorescent microscopic observations. All mitochondria in these mutants were longer and/or larger than wild-type mitochondria. The approximate chromosomal loci of the mutations of seven mutants that grew well were determined. The mitochondrial phenotypes of six of the mutants were recessive but the mitochondrial phenotype of the seventh mutant was dominant. Chromosomal rough mapping of the seven mutants showed that the mutations occurred at four different loci. At least one of these loci was novel, i.e., it was different from loci of other known mitochondrial morphology mutants of Arabidopsis and different from loci of Arabidopsis homologues of yeast genes related to mitochondrial morphology.<br>

Journal

• Genes & Genetic Systems

  Genes & Genetic Systems 79 (5), 301-305, 2004

  The Genetics Society of Japan

Keywords

• <i>Arabidopsis thaliana</i>
• chromosomal mapping
• mitochondrial fission
• mitochondrial morphology mutants

Details

• CRID: 1390001205473800064
• NII Article ID: 10014217551
• NII Book ID: AA11077421
• DOI: 10.1266/ggs.79.301
• ISSN: 18805779
• ISSN: 13417568
• NDL BIB ID: 7186251
• PubMed: 15599060
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7186251
• Web Site: http://www.jstage.jst.go.jp/article/ggs/79/5/79_5_301/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed