Derivation and morphological characterization of mouse spermatogonial stem cell lines

DOI Web Site PubMed 被引用文献16件 参考文献40件
  • Ogawa Takehiko
    Department of Urology, Yokohama City University Graduate School of Medicine
  • Ohmura Masako
    Department of Cell Differentiation, Keio University School of Medicine
  • Tamura Yoichi
    Department of Urology, Yokohama City University Graduate School of Medicine
  • Kita Kaoru
    Department of Urology, Yokohama City University Graduate School of Medicine
  • Ohbo Kazuyuki
    Department of Cell Differentiation, Keio University School of Medicine
  • Suda Toshio
    Department of Cell Differentiation, Keio University School of Medicine
  • Kubota Yoshinobu
    Department of Urology, Yokohama City University Graduate School of Medicine

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Spermatogonial stem cells (SSCs), having yet to possess decisive markers, can only be detected retrospectively by transplantation assay. It was reported recently that mouse gonocytes collected from DBA/2 and ICR neonates propagated in vitro. This cultured germ cell, named the germline stem cell (GS cell), produced functional sperm to make progeny when transplanted into recipient mouse testes. Here we show that GS cell lines can be established not only from neonatal testes but also from the testis of adult mice. We also confirmed that GS cells once transplanted into a host testis can be recovered to resume in vitro expansion, indicating that they are convertible mutually with SSCs in adult testes. Confocal laser microscopic examination showed GS cells resemble undifferentiated spermatogonia in the adult testis. This unique cell line could be useful for research in germ cell biology and applicable as a new tool for the genetic engineering of animals.

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