Failure to assemble the peri-nuclear structures in GOPC deficient spermatids as found in round-headed spermatozoa

Access this Article

Search this Article

Author(s)

    • ITO Chizuru
    • Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University
    • SUZUKI TOYOTA Fumie
    • Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University
    • MAEKAWA Mamiko
    • Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University
    • TOYAMA Yoshiro
    • Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University
    • YAO Ryoji
    • Department of Cell Biology, Japanese Foundation for Cancer Research (JFCR) Cancer Institute
    • NODA Tetsuo
    • Department of Cell Biology, Japanese Foundation for Cancer Research (JFCR) Cancer Institute
    • TOSHIMORI Kiyotaka
    • Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University

Abstract

Deletion of the <I>GOPC</I> gene encoding mouse GOPC (Golgi-associated PDZ- and coiled-coil motif-containing protein) causes infertile round-headed spermatozoa, which have acrosome-less round heads and deformed tails (Yao <I>et al</I>., 2002). This study investigated how <I>GOPC</I> deficient spermatids fail to assemble the peri-nuclear structures in round-headed spermatozoa during spermiogenesis in <I>GOPC</I> knockout mouse testes.<BR> In step 1-8 spermatids, Golgi-derived proacrosomal vesicles that are transported to the perinuclear region formed acrosome-like vesicles of various sizes, called pseudoacrosomes. The marginal ring of the acroplaxome, which is generally formed between the descending edge of a developing acrosome and nuclear envelope in a wild spermatid, was poorly formed between the pseudoacrosome and nuclear envelope. In step 9-11 elongating spermatids, a majority of pseudoacrosomes were detached from the nucleus and disappeared from the perinuclear region by spermiation. Concomitantly, several failures occurred on the nucleus, manchette, postacrosomal sheath (perinuclear theca), and posterior ring. Ectoplasmic specializations were poorly formed, and did not always associate with developing spermatids. Consequently, spermatid nuclear elongation to form round-headed spermatozoa developed was impaired.<BR> In addition to these sequential failures, the posterior ring deficiency was attributed to the tail deformation destined to occur during epididymal maturation as reported in an accompanying paper (Suzuki-Toyota <I>et al</I>., 2004 in this issue), its eventual phenotype being reminiscent of the round-headed spermatozoa of human infertile globozoospermia.

Journal

  • Archives of Histology and Cytology

    Archives of Histology and Cytology 67(4), 349-360, 2004-11-01

    International Society of Histology and Cytology

References:  34

Codes

  • NII Article ID (NAID)
    10014259556
  • NII NACSIS-CAT ID (NCID)
    AA1068990X
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09149465
  • Data Source
    CJP  J-STAGE 
Page Top