Induction of COX-2 in lapine synovial fibroblasts by uni-axial mechanical stretch

DOI 25 References
  • KAWAKAMI Tetsuji
    Department of Oral and Maxillofacial Surgery, Nara Medical University
  • MORISUGI Toshiaki
    Department of Oral and Maxillofacial Surgery, Nara Medical University
  • OHEDA Naoyuki
    Department of Oral and Maxillofacial Surgery, Nara Medical University
  • FUJITA Hirohito
    Department of Oral and Maxillofacial Surgery, Shimane Medical University
  • YOSHIMURA Yasuro
    Department of Oral and Maxillofacial Surgery, Shimane Medical University
  • KIRITA Tadaaki
    Department of Oral and Maxillofacial Surgery, Nara Medical University

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  • 伸展ストレスによるウサギ滑膜細胞のＣＯＸ－２発現誘導

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Abstract

It is proposed that synovial inflammation mediated by induced cyclooxygenase-2 (COX-2) plays an impor tantrole in the onset of temporomandibular joint (TMJ) pain. However, we have little knowledge about the pathophysiology of TMJ pain. In this study, the expression of COX-2 in lapine synovial fibroblast cells (HIG-82) following mechanical stretch was investi gated. Western blot analyses indicated that the level of the expression of COX-2 protein in HIG-82 in creasedin response to mechanical stretch (140%). We also observed nuclear translocation of p65 which is one of the nuclear factor-κ B (NF-κ B) family members in response to stretching. To investigate whether the induction of COX-2 was under NF-κ B control, we measured luciferase activity in cells transfected with pNF-κ B-Luc. Compared with the control, mechanical stretch treatment significantly increased the promoter activity of the NF-κ B. These results showed that mechanical stress on synovial membrane induced COX-2 via activation of NF-κ B. The present study suggested that synovial inflammation due to repetitive motion, such as bruxism, might lead to TMJ pain.

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