Detection Limit Estimated from Slope of Calibration Curve: An Application to Competitive ELISA

• HAYASHI Yuzuru

  National Institute of Health Sciences

• MATSUDA Rieko

  National Institute of Health Sciences

• ITO Katsutoshi

  School of Pharmaceutical Sciences, Showa University

• NISHIMURA Waka

  School of Pharmaceutical Sciences, Showa University

• IMAI Kazuhiro

  Research Institute of Pharmaceutical Sciences, Musashino University

• MAEDA Masako

  National Institute of Health Sciences

Abstract

This paper theoretically derives a general rule that while the slope of the semi-logarithmic plot (Y vs. log X) of a calibration curve varies depending on analyte concentration, X, the slope takes a specific value at the detection limit (L_D). This rule holds good irrespective of the shape of the calibration curve (linear or non-linear) and in this paper, is applied to competitive ELISA (enzyme linked immunosorbent assay). The following relationship is deduced: slope of log-dose B/B₀ at L_D = [relative standard deviation (RSD) of blank responses] ÷ 0.13. The L_D obtained from the above-mentioned slope corresponds to the dose at which the RSD of dose estimates is 0.3 (= 30%). A commercial kit for 17α-hydroxyprogesterone is taken as an example.

Journal

• Analytical Sciences

  Analytical Sciences 21 (2), 167-169, 2005

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204257982208
• NII Article ID: 10014365715
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.21.167
• COI: 1:CAS:528:DC%2BD2MXhs1emt7s%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 7242611
• PubMed: 15732478
• Web Site: http://id.ndl.go.jp/bib/7242611
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7242611
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.21.167.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.21.167/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/21/2/21_2_167/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed