Increased Levels of CTGF mRNA Expression in a Murine Model of Allergic Airway Inflammation

• Piao Hong Mei

• Yamauchi Kohei

  Third Department of Internal Medicine, Iwate Medical University School of Medicine

• Pan Li-Hua

  First Department of Pathology, Iwate Medical University School of Medicine

• Nakadate Toshihide

  Third Department of Internal Medicine, Iwate Medical University School of Medicine

• Ito Harumasa

  Third Department of Internal Medicine, Iwate Medical University School of Medicine

• Mouri Takashi

  Third Department of Internal Medicine, Iwate Medical University School of Medicine

• Kobayashi Hitoshi

  Third Department of Internal Medicine, Iwate Medical University School of Medicine

• Sawai Takashi

  First Department of Pathology, Iwate Medical University School of Medicine

• Nakanishi Tohru

  Department of Biochemistry and Molecular Dentistry, Okayama University Dental School

• Takigawa Masaharu

  Department of Biochemistry and Molecular Dentistry, Okayama University Dental School

• Inoue Hiroshi

  Third Department of Internal Medicine, Iwate Medical University School of Medicine

Abstract

Background: Connective tissue growth factor (CTGF) is known to play a direct role in fibrosis in various organs as a downstream mediator of TGF-β.<br> Objective: To evaluate a role in subepithelial fibrosis in the asthmatic airway, we investigated CTGF mRNA expression and CTGF producing cells in the airways of a murine asthma model with allergic inflammation.<br> Methods: After repetitive inhalation challenges with ovalbumin (OVA), cell numbers and TGF-β1 concentrations in bronchoalveolar lavage fluid from immunized mice were measured. Collagen deposition in lung tissue was estimated by measuring hydroxyproline content. CTGF mRNA and GAPDH mRNA levels were determined by quantitative RT-PCR method. Immunohistochemistry for CTGF with anti-CTGF antibody was performed.<br> Results: Numbers of eosinophils and TGF-β1 concentration increased markedly in BALF on the 7^th day and 14^th day after inhalation challenge with OVA. Hydroxyproline content in lung tissue increased significantly on the 14^th day after inhalation challenge of OVA compared to control. The ratio of CTGF mRNA /GAPDH mRNA in lung tissue in mice exposed to OVA increased 10-fold compared to those exposed to saline. Immunohistochemistry revealed that the number of CTGF-positive cells increased in bronchial submucosa after inhalation challenge of OVA.<br> Conclusions: Our results suggested that CTGF might be one of the potential molecules involved in subepithelial fibrosis in murine airways with allergic inflammation.<br>

Journal

• Allergology International

  Allergology International 54 (1), 107-115, 2005

  Japanese Society of Allergology

Keywords

• asthma
• collagen
• remodeling
• subepithelial fibrosis
• TGF-β

Details

• CRID: 1390282679607929088
• NII Article ID: 10014468908
• NII Book ID: AA11091750
• DOI: 10.2332/allergolint.54.107
• ISSN: 14401592
• ISSN: 13238930
• Web Site: http://api.elsevier.com/content/article/PII:S1323893015310121?httpAccept=text/xml
• Web Site: http://api.elsevier.com/content/article/PII:S1323893015310121?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2006305142
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed