Levels of spinorphin in cerebrospinal fluid derived from patients with pain increase with decreasing dipeptidyl peptidase III

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Author(s)

    • YAMAMOTO Yukio
    • Department of Medical Biology, Tokyo Metropolitan Institute of Medical Science
    • SATO Hiroshi
    • Department of Anesthesiology, Tokyo Metropolitan Komagome Hospital
    • FUKASAKU Susumu
    • Department of Orthopaedic Surgery, School of Medicine, Juntendo University
    • HIRAMATSU Kyoko
    • Department of Medical Biology, Tokyo Metropolitan Institute of Medical Science
    • TAI Sigeyuki
    • Department of Anesthesiology, Tokyo Metropolitan Bokutoh Hospital
    • SHIMAMURA Mariko
    • Department of Medical Biology, Tokyo Metropolitan Institute of Medical Science
    • HAZATO Tadahiko
    • Department of Medical Biology, Tokyo Metropolitan Institute of Medical Science

Abstract

   Spinorphin (LVVYPWT), an endogenous bioactive peptide purified from the spinal cord, demonstrates a nociceptive effect on bradykinin-induced pain in the rat and inhibitory activity against carrageenan-inducedneutrophil accumulation in the mouse model of acute inflammation. To further clarify its physiologicalrole in pain and inflammation, we quantified spinorphin in human cerebrospinal fluid (CSF) bycompetitive enzyme immunoassay (EIA) and analyzed the relation between its level and the physiological state. Levels of spinorphin measured in CSFs derived from patients with various types of pain other thancancer pain were higher in the pain group than in the controls (patients with pain-free and noninflammatory disease of the central nervous system) (21.4 ± 14.0 ng/ml, n=48 vs 8.61 ± 7.6 ng/ml, n=28, P<0.05). Furthermore, the activity of several spinorphin-processing enzymes that regulate some types of pain wasexamined. The activity of dipeptidyl peptidase III (DPPIII), an enkephalin-degrading enzyme, in CSFs ofthe pain group was lower than that in the control (l.40 ± 0.65 vs 2.05 ± 0.88 pmol / 30 min / 100 µl, P<0.05). Interestingly, a statistical moderate correlation was observed between the increase of spinorphin levels andthe decrease of DPPIII (r= -0.514, n=26). These results indicate that the level of spinorphin may, throughthe regulatory effect of enzymes, be an indicator of the pathophysiological state.

Journal

  • PAIN RESEARCH

    PAIN RESEARCH 20(1), 27-34, 2005-02-28

    JAPANESE ASSOCIATION FOR STUDY OF PAIN

References:  35

Codes

  • NII Article ID (NAID)
    10015444541
  • NII NACSIS-CAT ID (NCID)
    AN10195934
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09158588
  • Data Source
    CJP  J-STAGE 
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